spp1 (R&D Systems)
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Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+opn/Mouse+Osteopontin%2FOPN+Antibody/pmc13078873-293-69-71
Average 96 stars, based on 433 article reviews
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1) Product Images from "Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis"
Article Title: Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis
Journal: The Journal of Clinical Investigation
doi: 10.1172/JCI197556
Figure Legend Snippet: ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Techniques Used: Expressing, Quantitative RT-PCR, Infection
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