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spp1  (R&D Systems)


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    Structured Review

    R&D Systems spp1
    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of <t>SPP1,</t> SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+opn/Mouse+Osteopontin%2FOPN+Antibody/pmc13078873-293-69-71
    Average 96 stars, based on 433 article reviews
    spp1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis"

    Article Title: Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI197556

    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Infection

    Related Articles

    Incubation:

    Article Title: Loss and rescue of osteocalcin and osteopontin modulate osteogenic and angiogenic features of mesenchymal stem/stromal cells.
    Article Snippet: Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York Department of Bioengineering and iBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal Department of Chemistry and Chemical Biology, Biological Sciences and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York Department of Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, New York

    Article Title: Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/106/4/1232.full.html Updated information and services can be found at: (3230 articles) Hematopoiesis and Stem Cells (790 articles) Cell Adhesion and Motility Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:

    Staining:

    Article Title: A Novel Role for Osteopontin in Macrophage-Mediated Amyloid-β Clearance in Alzheimer’s Models
    Article Snippet: After fixation, cells were permeabilized with 2ml Saponin buffer (0.1% w/v Saponin # S4521, Sigma, 0.05% w/v NaN 3 , #438456, Sigma, in Hank’s Balanced salt solution sterile 1×, Corning) for 5 min. .. Samples were centrifuged and cells were stained for intracellular markers with the following antibody: PE-conjugated anti-mouse OPN (#IC808P, R&D). ..

    Labeling:

    Article Title: Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/106/4/1232.full.html Updated information and services can be found at: (3230 articles) Hematopoiesis and Stem Cells (790 articles) Cell Adhesion and Motility Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:



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    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of <t>SPP1,</t> SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis

    doi: 10.1172/JCI197556

    Figure Lengend Snippet: ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Immunoblotting was achieved using the indicated antibodies: phospho-IR Y1152/Y1153 (3024; Cell Signaling Technology [CST]), IR (sc-711), phospho-AKT S473 (4060; CST), phospho-AKT T308 (4056; CST), AKT (4685; CST), phospho-IR T1150 (NBP2-60777; Novus Biologicals), phospho-IRS1 S1097 (2385; CST), phospho-JNK (4668; CST), JNK (9252; CST), CHAC1 (ab76386, Abcam), Vinculin (MAB3574, Sigma), FASN (ab22759, Abcam), SCD1 (2438, CST), total OXPHOS (ab110413, Abcam), NOTCH1 (3608, CST), NOTCH2 (5732, CST), HES1 (sc-166410, Santa Cruz Biotechnology), SPP1 (AF808, R&D Systems), and β-tubulin (2146; CST).

    Techniques: Expressing, Quantitative RT-PCR, Infection